9 February 2025 The Future is the Product of the Past

DNA from 20,000-year-old deer-tooth pendant reveals woman who wore it

A pendant made of a deer tooth that was exposed to DNA about 20,000 years ago has yielded clues about the ancient woman who wore it.

The tooth, which was worn as a necklace bead, most likely absorbed DNA from the person’s sweat as it rubbed up against her chest and neck. Marie Soressi and her colleagues at Leiden University in the Netherlands were able to extract that DNA without damaging the tooth using a new process that took eight years to develop.

The technique might reveal unprecedented details about the social customs and gender roles of ancient populations, says Soressi.

The pendant was unearthed in Denisova Cave in Siberia, Russia, which was occupied by various species of hominin over 300,000 years. The study was published in Nature today.

“It’s almost like you open a time travel machine,” says study co-author Elena Essel, a molecular biologist who works on ancient DNA at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany. “With each sample we are able to learn a bit more and make more inferences about how these people lived.”

Previously, it was impossible to link tools and jewelry to the people who used them unless the artifact was discovered near a specific burial.

The entrance to Denisova cave, where the pendant was discovered. Photo: Richard G. Roberts
The entrance to Denisova cave, where the pendant was discovered. Photo: Richard G. Roberts

Essel and her colleagues coaxed DNA from inside the pores of the ancient pendant by soaking it in increasingly warm salt solution, which they found did not alter the pendant. Once released, the small amounts of genetic material were sequenced and compared with other sets of ancient DNA.

Mitochondrial DNA — which is handed down from mother to offspring — extracted from the pendant show that the object is roughly 19,000 to 25,000 years old and that the tooth belonged to a wapiti, also known as an elk (Cervus canadensis). Analysis of nuclear DNA from the ornament suggests that it had been made or worn by a female Homo sapiens whose genetic make-up resembles that of north Eurasian individuals who lived around the same time but were previously known only from remains found farther east in Siberia.

Study co-author Elena Zavala, a geneticist now at the University of California, Berkeley, says that the technique can connect ancient humans to “the tools that they created” — but, unlike other methods, does not destroy the artifact.

However, there are a number of drawbacks to the method. Modern DNA can easily contaminate an artifact, making analysis difficult. And even for the cleanest specimens, the DNA extraction method is time-consuming, requires sophisticated technology, and must be performed in a specialized laboratory. Essel and her colleagues are working to streamline their process. In the meantime, archaeologists can minimize contamination by wearing gloves and a face mask, and by immediately popping specimens into a refrigerator.

Still, Ludovic Orlando, a molecular archaeologist at the University of Paul Sabatier in Toulouse, France, who was not involved in the study, was impressed by the thoughtfulness and efficiency of the team’s procedure. The technique is not “a magic bullet”, says Orlando. But it still provides “a signature from nothing, from a piece of bone or tooth”, he adds. “You see the population signature of the people who interacted with the animal.”

doi: https://doi.org/10.1038/d41586-023-0482-3

Cover Photo: Max Planck Institute for Evolutionary Anthropology

Related Articles

Headless skeletons discovered in Prehistoric mass grave

14 January 2023

14 January 2023

Archaeologists have found a mass grave site containing 38 decapitated burials at a Neolithic settlement in Vráble, Slovakia. The remains...

More than 1,300 prehistoric burial mounds in western Azerbaijan systematically surveyed for the first time

2 January 2025

2 January 2025

Over 1,300 archaeological sites in Azerbaijan were systematically surveyed and documented in two field campaigns in 2021 and 2023 by...

Roman camp of 10,000 people discovered in northern Portugal

2 July 2021

2 July 2021

A camp used by 10,000 Roman soldiers sent to conquer northwestern Iberia has been discovered in the Portuguese city of...

A 4000-Year-Old Trading Port was Discovered in Istanbul

4 May 2021

4 May 2021

Archaeological excavations carried out on a peninsula in the middle of Istanbul Küçükçekmece Lake unearthed a very important 4,000-year-old trade...

Horse cemetery in Westminster revealed as likely resting place for elite imported animals

25 March 2024

25 March 2024

Archaeological analysis of a medieval horse cemetery discovered in London nearly 30 years ago has revealed the international scale of...

The Latest Surprises Revealed by Investigations Inside the Tomb of Cerberus in Giugliano

24 July 2024

24 July 2024

The latest surprise revealed by investigations at the Tomb of Cerberus in Giugliano: The remains of a corpse covered with...

4,500-Year-Old Three Warrior Graves Found in Germany, One Still Wearing an Arm Guard

30 January 2025

30 January 2025

Extraordinary discovery during the construction of a New Power Line: Archaeologists unearth a cemetery from the Copper Age with Three...

The Famous Cueva de Ardales cave in Spain was used by ancient humans for over 50,000 years

8 June 2022

8 June 2022

Cueva de Ardales cave in Málaga, Spain,  famed for the extensive prehistoric art on its walls was excavated for the...

Large Roman Complex found in Swiss Gravel Quarry

30 August 2023

30 August 2023

Archaeologists have discovered the remains of walls of a Roman building complex built nearly 2,000 years ago in the Äbnetwald...

Unique semi-mummified body tomb discovered in Pompeii

17 August 2021

17 August 2021

A semi-mummified skeleton was discovered in the Porta Sarno necropolis, which is located east of Pompeii’s city center and is...

Archaeologists have discovered the remains of a stone circle in the Castilly Henge, located in Cornwall, England

20 May 2022

20 May 2022

Archaeologists have unearthed a mysterious stone circle at the center of a prehistoric ritual site near Bodmin in Cornwall, located...

Scientists reconstruct Late Bronze and Iron Age Mediterranean silver trade

11 July 2021

11 July 2021

Scientists have recreated the Eastern Mediterranean silver trade across a time span that includes the conventional dates of the Trojan...

Researcher found the head of the statue of Bacchus, inside a water channel near the ancient city of Cyrene in Libya

31 December 2023

31 December 2023

Libyan Archeology researcher, Issam Menfi found the head of the statue of Bacchus, which dates back to the Greek era,...

7,800-year-old female figurine discovered in Ulucak Höyük in western Turkey

8 August 2022

8 August 2022

A 7,800-year-old female figurine was found in the Ulucak Höyük (Ulucak Mound) in the Kemalpaşa district of Izmir. It was...

Iznik Archaeology Museum reveals 2,500-year-old love letter

16 January 2023

16 January 2023

İznik is an ancient habitation that hosts various civilizations due to its fertile lands, trade routes, and many other reasons....